Mchonde, Gabriel J.Satoh, Yoh-ichiSaino, Tomoyuki2023-05-222023-05-222015Mchonde, G. J., Satoh, Y. I., & Saino, T. (2015). ATP-Induced Nuclear and Cytosolic Ca 2 Signal in Various Stages of Interphase HeLa/Fucci2 Cell. Int. J. Sci.: Basic and Applied Res, 21, 274-286.URL: https://core.ac.uk/download/pdf/249334585.pdfhttp://hdl.handle.net/20.500.12661/3779Abstract. Full text article is available at https://core.ac.uk/download/pdf/249334585.pdfCell cycle progression involves the coordination of growth, DNA replication and division in proliferating cells. These intracellular activities are linked with calcium signaling in which calcium ion (Ca2+) act as a second messenger or as an agent for phosphorylation processes. Previous studies have examined the Ca2+ in the dividing cells; however, none of these gave the detailed explanation on Ca2+ dynamics in an interphase cell. This study addresses here the issue of whether the nuclear and cytosolic Ca2+ concentrations ([Ca2+]n and [Ca2+]c) changes are related or not in the dividing interphase cells. Current approach used interphase HeLa/Fucci2 cells to investigate the intracellular Ca2+ dynamics between the nucleoplasm and cytoplasm following stimulation with extracellular adenosine triphosphate (extracellular purinergic-2 receptor agonists). The results revealed that [Ca2+]n and [Ca2+]c signals differed both between and within interphase stages.enATP-induced nuclearcytosolic ca2+ signalDNA replicationCell cycle progressionInterphase hela/fucci2 cellIntracellular Ca2+ dynamicsExtracellular adenosine triphosphateArticle