Patova, O. ALuаnda, APaderin, N. MPopov, S. VMakangara, J. JKuznetsov, S. PKalmykova, E. N2022-03-082022-03-082021Patova, O. A., Luаnda, A., Paderin, N. M., Popov, S. V., Makangara, J. J., Kuznetsov, S. P., & Kalmykova, E. N. (2021). Xylogalacturonan-enriched pectin from the fruit pulp of Adansonia digitata: Structural characterization and antidepressant-like effect. Carbohydrate Polymers, 262.DOI: 10.1016/j.carbpol.2021.117946http://hdl.handle.net/20.500.12661/3453Abstract. Full text article available at: https://doi.org/10.1016/j.carbpol.2021.117946The low methyl-esterified and acetylated xylogalacturonan (DM 20 %, DA 2 %, Mw ∼ 58 kDa) was isolated by water extraction for 4 h × 2 at 50 °C (yield 23 %) from the pulp of baobab fruit (Adansonia digitata L.). Subsequent tightening of the conditions for water extraction by mean increasing the temperature to 70 °C and time to 12 h led to the co-extraction of small amounts of starch components and RG I with xylogalacturonan. Structural analysis (DEAE-cellulose ion-exchange chromatography, HPSEC, monosaccharide analysis, NMR spectroscopy) revealed that about 12 mol. % of 1,4-linked α-GalpA residues were substituted by single β-Xylp residues at the O-3 position. The xylogalacturonan was found to possess an antidepressant-like effect in mice. The study offers using the baobab fruit as a rich source of soluble dietary fiber – water-soluble pectin with beneficial physiological effect.enBaobabBiological activityNMRPolysaccharide isolationArticle