Identification of large variation in pfcrt, pfmdr-1 and pfubp-1 markers in Plasmodium falciparum isolates from Ethiopia and Tanzania

Simple item page
dc.contributor.authorGolassa, L.
dc.contributor.authorKamugisha, E.
dc.contributor.authorIshengoma, D. S.
dc.contributor.authorBaraka, V.
dc.contributor.authorShayo, A.
dc.contributor.authorBaliraine, F. N.
dc.contributor.authorSwedberg, G.
dc.date.accessioned2020-03-23T08:34:14Z
dc.date.available2020-03-23T08:34:14Z
dc.date.issued2015
dc.descriptionFull Text Article. Also available at: 10.1186/s12936-015-0783-3en_US
dc.description.abstractPlasmodium falciparum resistance to anti-malarials is a major drawback in effective malaria control and elimination globally. Artemisinin-combination therapy (ACT) is currently the key first-line treatment for uncomplicated falciparum malaria. Plasmodium falciparum genetic signatures at pfmdr-1, pfcrt, and pfubp-1 loci are known to modulate in vivo and in vitro parasite response to ACT. The objective of this study was to assess the distribution of these resistance gene markers in isolates collected from different malaria transmission intensity in Ethiopia and Tanzania Plasmodium falciparum clinical isolates were collected from different regions of Ethiopia and Tanzania. Genetic polymorphisms in the genes pfcrt, pfmdr-1 and pfubp-1 were analysed by PCR and sequencing. Frequencies of the different alleles in the three genes were compared within and between regions, and between the two countries. The majority of the isolates from Ethiopia were mutant for the pfcrt 76 and wild-type for pfmdr-1 86. In contrast, the majority of the Tanzanian samples were wild-type for both pfcrt and pfmdr-1 loci. Analysis of a variable linker region in pfmdr-1 showed substantial variation in isolates from Tanzania as compared to Ethiopian isolates that had minimal variation. Direct sequencing of the pfubp-1 region showed that 92.8% (26/28) of the Ethiopian isolates had identical genome sequence with the wild type reference P. falciparum strain 3D7. Of 42 isolates from Tanzania, only 13 (30.9%) had identical genome sequences with 3D7. In the Tanzanian samples, 10 variant haplotypes were identified. The majority of Ethiopian isolates carried the main marker for chloroquine (CQ) resistance, while the majority of the samples from Tanzania carried markers for CQ susceptibility. Polymorphic genes showed substantially more variation in Tanzanian isolates. The low variability in the polymorphic region of pfmdr-1 in Ethiopia may be a consequence of low transmission intensity as compared to high transmission intensity and large variations in Tanzaniaen_US
dc.identifier.citationGolassa, L., Kamugisha, E., Ishengoma, D. S., Baraka, V., Shayo, A., Baliraine, F. N., ... & Swedberg, G. (2015). Identification of large variation in pfcrt, pfmdr-1 and pfubp-1 markers in Plasmodium falciparum isolates from Ethiopia and Tanzania. Malaria Journal, 14(1), 264.en_US
dc.identifier.other10.1186/s12936-015-0783-3
dc.identifier.urihttp://hdl.handle.net/20.500.12661/2282
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectMalariaen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectPfcrten_US
dc.subjectPfmdr-1en_US
dc.subjectPfubp-1en_US
dc.subjectEthiopiaen_US
dc.subjectTanzaniaen_US
dc.subjectArtemisinin Combination Therapyen_US
dc.subjectACTen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectIn vitro parasiteen_US
dc.subjectIn vivo parasiteen_US
dc.subjectChloroquine resistance transporter geneen_US
dc.titleIdentification of large variation in pfcrt, pfmdr-1 and pfubp-1 markers in Plasmodium falciparum isolates from Ethiopia and Tanzaniaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Golassa2015_.pdf
Size:
1.13 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles