Browsing by Author "Saino, Tomoyuki"

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    ATP-induced nuclear and cytosolic ca2+ signal in various stages of interphase hela/fucci2 cell
    (Mohammad Nassar for Researches (MNFR), 2015) Mchonde, Gabriel J.; Satoh, Yoh-ichi; Saino, Tomoyuki
    Cell cycle progression involves the coordination of growth, DNA replication and division in proliferating cells. These intracellular activities are linked with calcium signaling in which calcium ion (Ca2+) act as a second messenger or as an agent for phosphorylation processes. Previous studies have examined the Ca2+ in the dividing cells; however, none of these gave the detailed explanation on Ca2+ dynamics in an interphase cell. This study addresses here the issue of whether the nuclear and cytosolic Ca2+ concentrations ([Ca2+]n and [Ca2+]c) changes are related or not in the dividing interphase cells. Current approach used interphase HeLa/Fucci2 cells to investigate the intracellular Ca2+ dynamics between the nucleoplasm and cytoplasm following stimulation with extracellular adenosine triphosphate (extracellular purinergic-2 receptor agonists). The results revealed that [Ca2+]n and [Ca2+]c signals differed both between and within interphase stages.
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    Variant position and course of the superior cervical Cardiac branch of vagus nerve
    (IMED Research Publications, 2017) Mchonde, Gabriel J.; Saino, Tomoyuki
    Variations on course and position of vagus nerve and its branches are extremely rare congenital anomaly. This manuscript presents a case of left superior cervical cardiac branch of the vagus nerve looping over the subclavian vein observed during routine dissection of a 72-yeays-old embalmed caucasian female cadaver. In the same case, the left inferior cervical cardiac branch was taking origin from the uperior cervical branch. Understanding the existence and developmental variations of these two cervical cardiac branches is of important in clinical presentations.